Hasan Ravansalar; MohammadJavad Namazi; Hossein Moallaei; Arash Akaberi
Volume 17, Issue 1 , March and April 2010, , Pages 54-62
Abstract
Background and Purpose: Vulvovaginal candidiasis is one of the most common infections in women and is caused by various types of candidiasis with different patterns of resistance against drugs. The present study was conducted to determine the various species of candidiasis in women admitted to Mobini ...
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Background and Purpose: Vulvovaginal candidiasis is one of the most common infections in women and is caused by various types of candidiasis with different patterns of resistance against drugs. The present study was conducted to determine the various species of candidiasis in women admitted to Mobini Hospital in Sabzevar, Iran in order to make preventive recommendations for medical doctors. Methods and Materials: This cross-sectional descriptive analytical research was conducted on 231 women admitted to Mobini Hospital in Sabzevar, Iran, complaining of vaginal secretions. After getting the ethics committee permissions and obtaining the informed consent of the participants, at the same time as measuring vaginal pH, two swaps were used for collecting samples of secretions from lateral and posterior fornix. They were examined for yeasts and were then cultured in saburu dextrose. Grown yeasts were detected based on their macroscopic and microscopic features, tubulation in blood serum and biochemical characteristics using Yeast Plus System. The obtained data were analyzed in SPSS 11 using chi-square and bi-dimensional tables. Results: Positives results in direct examination results were 7.35%, and in culture the positive results were 26.8%. In detecting processes, candida albicans yeast (38.7%), Candida kefyr (17.7%), Saccharomyces cerevisiae (14,5%); candia tropicalis (9.6%), Candida glabrata (8%), candida cruse (3.2%) were isolated with other non-pathogenic yeasts including Candida rugosa, Candida lipolytica, trycospron bejli, Blastoschizomyces capitatus (6.4%). Conclusion: Non-pathogenic yeasts were isolated from patients with defective immunity systems, and need to be considered in patients with vaginal infections.
Ciristina Verissimo; Joua Brando; Hossein Moallaie; Laura Rosado
Volume 16, Issue 4 , January and February 2010, , Pages 213-219
Abstract
Background and Purpose: Due to the ever-increasing use of antifungal drugs especially those of azole group, the prevalence of recurrent forms of vaginal infections and the number of drug-resistant yeasts are on the rise. Therefore, the rpesent study is conducted to investigate the sensitivity and resistance ...
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Background and Purpose: Due to the ever-increasing use of antifungal drugs especially those of azole group, the prevalence of recurrent forms of vaginal infections and the number of drug-resistant yeasts are on the rise. Therefore, the rpesent study is conducted to investigate the sensitivity and resistance of yeasts isolated from vaginal infections to antifungal drugs.
Methods and Materials: This cross-sectional descriptive analytical study was conducted on 118 yeasts isolated from 436 suspects of vulvovaginal candidiasis; their sensitivity and resisitance to drugs belonging to imidazoles group including clotrimazole (CTR), fluconazole (FCA), ketoconazole (KET), miconazole (MCZ) and econazole (EC) as well as nystatine (NY) belonging to polyene group using the standard disc diffusion technique. To determine their relationship clinical symptoms and the raltion of resistance to one drug with resistance to other drugs, relevant tests were used including chi-square, kappa and linear regression coefficient in SPSS 11.
Results: The results showed no resistance to nystatine from polyene group and econazole from azole group; however, 53 cases (%44.9) were resistant to fluconazole, 26 cases (%22) to miconazole, 10 cases (%8.5) to clotrimazole and 2 cases (%1.7) to ketoconazole.
Conclusion: The results of linear correlation showed a negative correlation between the sensitivity of yeasts to CTR and KET and clinical symptoms.
Jerald Larcher; Mahmood Mahmoodi; Farideh Zeini; Hossein Moallaei; Seyyed Mehdi Zargariyan
Volume 15, Issue 3 , September and October 2008, , Pages 169-175
Abstract
Background and Purpose: Keratinolytic fungi (KF) are among the fungi existing in the soil whose enzymatic activity causes the decomposition of keratinized material in the soil; they are also considered as one of the pathogenic factors. The present study was conducted to determine the enzymatic activity ...
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Background and Purpose: Keratinolytic fungi (KF) are among the fungi existing in the soil whose enzymatic activity causes the decomposition of keratinized material in the soil; they are also considered as one of the pathogenic factors. The present study was conducted to determine the enzymatic activity of the KF and to evaluate the effect of climatic conditions on their activity. Methods and Materials: This deh1ive cross-sectional study was conducted on 90 isolates of KF randomly selected from different regions in Iran and other countries in 2004. The samples were cultured in Modified Czaspex-dextrose liquid medium (MCDLM). Their enzymatic activity was measured using Azocazein the general subtilisin of serin proteinase and chromogenic substrates including N-Suc-Ala-Ala-Pro-Phe-ρNA N-Suc-Ala-Ala-Pro-Leu-ρNA and N-Bz- Phe-Val-Arg- ρNA. The obtained data were summarized using two-dimensional tables and were analyzed using non-repeating one-way ANOVA and Tukey test in SPSS. Results: The results indicated that Myriodontium keratinophilum (94.6 u/ml) and Microsporum coockei (81.66 u/ml) had the highest Azocaseinolytic activity and hydrolysed N-Suc-Ala-Ala-Pro-Phe-ρNA N-Bz- Phe-Val-Arg- ρNA and N-Suc-Ala-Ala-Pro-Leu-ρNA respectively. Statistically significant differences were onserved to exist between methods of determining enzymatic activity (P
M MAHMOUDI; F ZEYNI; H MOALAEI; J HASHEMI; M PIT
Volume 13, Issue 2 , July and August 2006, , Pages 64-73
Abstract
Background and purpose: Keratinophilic fungi are ecologically, medically and industrially important and farming soil is suitable for their growth due to their keratin and fertilizers. Therefore, this study was intended to determine the different types of keratinophilic fungi in dry-farming soil samples.
Methods ...
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Background and purpose: Keratinophilic fungi are ecologically, medically and industrially important and farming soil is suitable for their growth due to their keratin and fertilizers. Therefore, this study was intended to determine the different types of keratinophilic fungi in dry-farming soil samples.
Methods and Materials: Using stratified sampling, this descriptive comparative study included 100 soil samples from dry-farming areas in South and Razavi Khorasan Provinces in Iran. Samples were cultured through Van beuseghemi method and various fungi within the cultures were identified and counted. Relevant data were analyzed using two-scale and multi-scale tables as well as statistical tests.
Results: 289 colonies were isolated which included 20 species in 16 genuses. Fusarium SP With 53 colonies (18.33%), Annixopsis stercoraria with 47 colonies (16.26%) and Aspergillus SP varieties with 44 colonies (15.22%) were found to be the most common species. Also, McNemar's test revealed A. stercoraria, F. oxyspayum and penicillium SP to be dominant in the area (P